Abstract:
Cauliflower exhibits a transition from vegetative to flowering growth triggered by
low temperature (vernalization) which controls late or early flowering. FLC gene
homologs in Brassica species controls flowering response. In this study, integrated
molecular and tissue culture approaches were used to explore genetic variation in
the flowering genes and to improved cauliflower crop. In the molecular approach,
part of the BoFLC.HP gene was sequenced in hybrid and local "Baladi" cauliflower
using the F7R7 primer to characterize early or late FLC gene in cauliflower.
Sequenced BoFLC.HP region showed a point mutation at position 91 and an indel at
position 212 to 219 in intron number 2 between Baladi and F1- hybrid cauliflower
which controls flowering time. In the tissue culture approach, hypocotyl and
cotyledon explants from four-day-old seedlings were cultured on Murashige and
Skoog (MS) salts supplemented with BA, KIN and TDZ alone or in combination with
IBA or NAA. The highest shoot regeneration response (80%) was observed in
hypocotyl explants on full strength MS media supplemented 0.5 mg/L BA and 0.5
mg/L IBA. Cotyledon explants, showed 26% shoot regeneration on full strength MS
media supplemented 2.0 mg/L BA and 0.1 mg/L NAA. One of the most important
tools in crop improvement is the process of genetic transformation with the
Agrobacterium, which requires an efficient in vitro shoot regeneration protocol from
explants. These two aspects will be a prerequisite in cauliflower crop development
in the future.
Keywords: Brassica oleracea var. botrytis, flowering time, vernalization, BoFLC.HP
gene, in vitro shoot regeneration, Hypocotyl, Cotyledon.
Description:
CD , no of pages 52, 31152, biotechnology 4/2021
