Allele frequencies of CYP2C9, CYP2C19, and CYP2D6 in the palesinian population

Abstract

The polymorphism of CYP2C9, CYP2C19 and CYP2D6 differs among different individuals and shows ethnic variability. This will affect the metabolism of several drugs and may cause differences in the clinical response. In this study, we analyzed the genotype profile of a random sample of the Palestinian population to compare the allele frequencies of CYP2C9, CYP2C19 and CYP2D6. Data have been taken from 67 unrelated Palestinian volunteers (35 males and 32 females, aged from 16 to 60 years). Next generation sequencing (NGS) was conducted to sequence the most frequent variants of the CYP2C9, CYP2C19, and CYP2D6. To compare the frequency of different variants with the global data, we used two libraries of python, i.e. Matplotlib, and Pandas. The frequency of CYP2C9 and CYP2D6 between the Palestinian population and the global populations shows a high correlation (0.95, and 0.96 respectively). However, we found a low correlation for CYP2C19 (0.34). Only rs9332239 and rs28371685 in CYP2C9 variants’ frequencies showed a significant difference between the Palestinian population and the global population, (p-value=0.00311776) for the reference variant and (pvalue=0.003118) for the alternative variant, respectively. For CYP2C19 variants: rs28399504 and rs41291556 showed a significant difference between the Palestinian population and the global population (p-value=0.014664745) for the reference variant and (p-value=0.014664745) for the alternative variant. There were no statistically significant differences for all the other variants examined for CYP2C9 (P-value = 0.847232), CYP2C19 (P-value= 0.479754), and CYP2D6 (P-value= 0.923857). Up to our knowledge this is the first study done to compare the Palestinian pharmacogenetics profile in those specific genes. Further studies on a larger population sample are necessary to confirm our findings