Detection Of Escherichia coli In Water Using Capacitance Biosensor

dc.contributor.advisorMujahed, Arwa
dc.contributor.authorMujahed, Arwa
dc.date.accessioned2025-08-30T10:23:29Z
dc.date.available2025-08-30T10:23:29Z
dc.date.issued2025-03-15
dc.description.abstractPathogenic bacteria that are the main causative agent for biological contamination of water have always posed one of the most serious threats to public health, and continue to be specifically dangerous with the rise in antibiotic resistance. The incidence of these infectious agents needs rapid, point-of-care and real-time sensors for their detection and monitoring. Therefore, continuous real-time monitoring provides better microbiological control of the water and helps prevent contaminated water from reaching the households. In this research we have developed a method to detect E.coli(Escherichia coli) bacteria in water depending upon a large scale capacitance biosensor in the presence and absence of E.colicells. First we show that the sensor is able to detect E. coli(Gram-negative) bacteria in water samples. Next, we demonstrate the sensor’s ability to measure the bacteria concentration suspended in water sample by comparing the results to those obtained by the traditional measurement using spectrophotometer. The results determine a correlation between the capacitance measurement andthe real bacterial concentration which indicate an inversely relationship between the bacterial concentration and the capacitance measurement.Our investigations show that the capacitance biosensor has the potential to be extremely effective at detecting sudden bacterial contaminations found in drinking water in real-time and it is promising for the low-cost monitoring of bacterial growth and the detection of specific E.coliconcentration due to the consistency and ease-of-use of capacitance measurementsen_US
dc.identifier.citationThe International Innovations Journal of Applied Science (IIJAS) Vol. 2, No.1, 15-03-2025en_US
dc.identifier.issn3009-1853
dc.identifier.urischolar.ppu.edu/handle/123456789/9244
dc.language.isoenen_US
dc.publisherThe International Innovations Journal of Applied Science (IIJAS)en_US
dc.subjectCapacitance biosensorsCapacitance Measuring circuit,E.coliConcentrationWater contamination,Bacteria detection1. IntroductionWith the increase in antibiotic resistance, pathogenic bacteria remain a significant and ongoing threat to public health. Consequently, there is a growing need for the development of rapid, real-time, point-of-care sensors to detect and monitor these infectious agents, given their widespread presence.One of the pathogens requiring monitoring is the Escherichia coli(E. coli) bacteria. The presence of these bacteria in water is associated with fatalities and serves as an indicator of the proliferation of other pathogens (Quirozet al.,2018). Many conventional techniques and methods are used to detect E.coli. Such techniques are sample culture(Lazcka et al.,2007), Polymerase Chain Reaction Methods (Toze., 1999), Immunology-Based Methods (Haglundet al.,1964). Most of the conventional methods of bacterial detection and identification have enabled the monitoring of real-world samples. These techniques are all limited by the time required and the necessary instrumentation, which can be costly and highly specialized, and the facilities and personnel required to complete these assays.Impedance-based techniques are some of the most reliable and reproducible characterization methods for electrical systems,en_US
dc.titleDetection Of Escherichia coli In Water Using Capacitance Biosensoren_US
dc.title.alternativeDetection Of Escherichia coli In Water Using Capacitance Biosensoren_US

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