DSpace Repository

Regulation of iron uptake in primary culture rat hepatocytes: the role of acute-phase cytokines

Show simple item record

dc.contributor.author Ahmad, Shakil
dc.contributor.author Sultan, Sadaf
dc.contributor.author Naz, Naila
dc.contributor.author Ahmad, Ghayyor
dc.contributor.author Alwahsh, Salamah M
dc.contributor.author Cameron, Silke
dc.contributor.author Moriconi, Federico
dc.contributor.author Ramadori, Giuliano
dc.contributor.author Malik, Ihtzaz A
dc.date.accessioned 2021-05-04T08:02:40Z
dc.date.accessioned 2022-05-22T08:55:32Z
dc.date.available 2021-05-04T08:02:40Z
dc.date.available 2022-05-22T08:55:32Z
dc.date.issued 2014
dc.identifier.uri https://pubmed.ncbi.nlm.nih.gov/24365882/
dc.identifier.uri http://localhost:8080/xmlui/handle/123456789/8361
dc.description.abstract Decreased serum and increased hepatic iron uptake is the hallmark of acute-phase (AP) response. Iron uptake is controlled by iron transport proteins such as transferrin receptors (TfRs) and lipocalin 2 (LCN-2). The current study aimed to understand the regulation of iron uptake in primary culture hepatocytes in the presence/absence of AP mediators. Rat hepatocytes were stimulated with different concentrations of iron alone (0.01, 0.1, 0.5 mM) and AP cytokines (interleukin 6 [IL-6], IL-1β, tumor necrosis factor α) in the presence/absence of iron (FeCl3: 0.1 mM). Hepatocytes were harvested at different time points (0, 6, 12, 24 h). Total mRNA and proteins were extracted for reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot. A significant iron uptake was detected with 0.1 mM iron administration with a maximum (133.37 ± 4.82 µg/g of protein) at 24 h compared with control and other iron concentrations. This uptake was further enhanced in the presence of AP cytokines with a maximum iron uptake (481 ± 25.81 µg/g of protein) after concomitant administration of IL-6 + iron to cultured hepatocytes. Concomitantly, gene expression of LCN-2 and ferritin subunits (light- and heavy-chain ferritin subunits) was upregulated by iron or/and AP cytokines with a maximum at 24 h both at mRNA and protein levels. In contrast, a decreased TfR1 level was detected by IL-6 and iron alone, whereas combination of iron and AP cytokines (mainly IL-6) abrogated the downregulation of TfR1. An increase in LCN-2 release into the supernatant of cultured hepatocytes was observed after addition of iron/AP cytokines into the medium. This increase in secretion was further enhanced by combination of IL-6 + iron. In conclusion, iron uptake is tightly controlled by already present iron concentration in the culture. This uptake can be further enhanced by AP cytokines, mainly by IL-6. en_US
dc.language.iso en en_US
dc.publisher Wolters Kluwer en_US
dc.subject Iron metabolism, hepatocyte en_US
dc.title Regulation of iron uptake in primary culture rat hepatocytes: the role of acute-phase cytokines en_US
dc.type Article en_US

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


My Account