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A one-tube polymerase chain reaction protocol demonstrates CC chemokine overexpression in Graves' disease glands.

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dc.contributor.author Ashhab, Y
dc.contributor.author Dominguez, O
dc.contributor.author Sospedra, M
dc.contributor.author Roura-Mir, C
dc.contributor.author Lucas-Martín, A
dc.contributor.author Pujol-Borrell, R
dc.date.accessioned 2020-11-28T07:04:11Z
dc.date.accessioned 2022-05-22T08:28:02Z
dc.date.available 2020-11-28T07:04:11Z
dc.date.available 2022-05-22T08:28:02Z
dc.date.issued 1999-08
dc.identifier 10.1210/jcem.84.8.5909
dc.identifier.issn 0021-972X
dc.identifier.uri http://localhost:8080/xmlui/handle/123456789/7897
dc.description.abstract An adaptation of mixed oligonucleotide primed amplification of complementary DNA to detect the profile of CC chemokines in biological samples is presented. By introducing normalization, two correction coefficients, performing a single amplification reaction, and five parallel hybridizations, intrasample and intersample comparisons can be reliably made. This protocol of single tube PCR CC chemokine profiling was applied to tissue samples from an autoimmune thyroid condition, Graves' disease, and from a nonautoimmune condition, multinodular goiter. Results demonstrate overexpression of CC chemokines in Graves' disease, statistically significant for macrophage inflammatory protein-1alpha and -1beta, which correlated with the aberrant human leukocyte antigen class II expression by thyrocytes, as assessed by flow cytometry. Overexpression of CC chemokines probably plays a major role in determining the characteristics of the lymphocytes migrating to the thyroid gland and influences the course of the disease. The study of chemokine profile should be more informative than the study of isolated chemokines and cytokines, and as it can be applied to fine needle aspiration biopsies, it may be useful to clinical research.
dc.language.iso eng
dc.source The Journal of clinical endocrinology and metabolism
dc.title A one-tube polymerase chain reaction protocol demonstrates CC chemokine overexpression in Graves' disease glands.
dc.type Journal Article
dc.type Research Support, Non-U.S. Gov't


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