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SslE elicits functional antibodies that impair in vitro mucinase activity and in vivo colonization by both intestinal and extraintestinal Escherichia coli strains.

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dc.contributor.author Nesta, Barbara
dc.contributor.author Valeri, Maria
dc.contributor.author Spagnuolo, Angela
dc.contributor.author Rosini, Roberto
dc.contributor.author Mora, Marirosa
dc.contributor.author Donato, Paolo
dc.contributor.author Alteri, Christopher J
dc.contributor.author Del Vecchio, Mariangela
dc.contributor.author Buccato, Scilla
dc.contributor.author Pezzicoli, Alfredo
dc.contributor.author Bertoldi, Isabella
dc.contributor.author Buzzigoli, Lapo
dc.contributor.author Tuscano, Giovanna
dc.contributor.author Falduto, Maria
dc.contributor.author Rippa, Valentina
dc.contributor.author Ashhab, Yaqoub
dc.contributor.author Bensi, Giuliano
dc.contributor.author Fontana, Maria Rita
dc.contributor.author Seib, Kate L
dc.contributor.author Mobley, Harry L T
dc.contributor.author Pizza, Mariagrazia
dc.contributor.author Soriani, Marco
dc.contributor.author Serino, Laura
dc.date.accessioned 2020-11-28T07:04:08Z
dc.date.accessioned 2022-05-22T08:28:00Z
dc.date.available 2020-11-28T07:04:08Z
dc.date.available 2022-05-22T08:28:00Z
dc.date.issued 2014-05
dc.identifier 10.1371/journal.ppat.1004124
dc.identifier.uri http://localhost:8080/xmlui/handle/123456789/7885
dc.description.abstract SslE, the Secreted and surface-associated lipoprotein from Escherichia coli, has recently been associated to the M60-like extracellular zinc-metalloprotease sub-family which is implicated in glycan recognition and processing. SslE can be divided into two main variants and we recently proposed it as a potential vaccine candidate. By applying a number of in vitro bioassays and comparing wild type, knockout mutant and complemented strains, we have now demonstrated that SslE specifically contributes to degradation of mucin substrates, typically present in the intestine and bladder. Mutation of the zinc metallopeptidase motif of SslE dramatically impaired E. coli mucinase activity, confirming the specificity of the phenotype observed. Moreover, antibodies raised against variant I SslE, cloned from strain IHE3034 (SslEIHE3034), are able to inhibit translocation of E. coli strains expressing different variants through a mucin-based matrix, suggesting that SslE induces cross-reactive functional antibodies that affect the metallopeptidase activity. To test this hypothesis, we used well-established animal models and demonstrated that immunization with SslEIHE3034 significantly reduced gut, kidney and spleen colonization by strains producing variant II SslE and belonging to different pathotypes. Taken together, these data strongly support the importance of SslE in E. coli colonization of mucosal surfaces and reinforce the use of this antigen as a component of a broadly protective vaccine against pathogenic E. coli species.
dc.language.iso eng
dc.source PLoS pathogens
dc.title SslE elicits functional antibodies that impair in vitro mucinase activity and in vivo colonization by both intestinal and extraintestinal Escherichia coli strains.
dc.type Journal Article
dc.type Research Support, N.I.H., Extramural
dc.type Research Support, Non-U.S. Gov't

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