Abstract:
Several studies implicated that contamination of poultry carcasses in abattoirs is
considered a significant source of human infections. Campylobacter, Salmonella,
E.coli and Staphylococcus aureus are among the high prevalent pathogens causing
foodborne diseases.
However, due to the random and inefficient use of antibiotics in poultry, such
pathogens are increasingly resistant to the clinically important antibiotics and this
rising resistance is a concern for public health. This study aimed to compare the
Presence and antimicrobial susceptibility of Campylobacter, Salmonella, E. coli and
S. aureus species in chicken carcasses.
Four samples were collected from a poultry farms in (Dora and Hebron), Bacteria
strains were isolated and recognized using selective and deferential media. Isolates
were then tested for sensitivity to Azithromycin, Ampicillin, Tetracycline,
Gentamicin, and Nalidixic Acid antibiotics using the Kirby-Bauer disk diffusion test
with reference of the Clinical and Laboratory Standards Institute (CLSI).
Results indicates the highest level of resistance among Campylobacter (n=19) tested
was to Nalidixic Acid (NA) 100%, Tetracycline and Azithromycin (AZM) 94%,It
showed moderate resistance to a Gentamycin 10.5 %, and Ampicillin 26.3%
While Salmonella (n=6) bacteria showed highly 100 % resistance to AM, NA, also
67 % to AZM. While its highly sensitive to CN antibiotic.
For S. aureus (n=9), disc diffusion testing showed 100% correlation with agar dilution
for (TE) and (NA), and 89% AZM. A high level of sensitivity 100% to AZM, CN
and 16.6% to AM was found in the E.coli isolates (n=6) by disc, whereas 83 % of
isolates were resistance to AM, 50% to NA and low level 33.3% to TE.
Overall, our study has emphasize on minimize the misuse of available antimicrobials
in agriculture and medicine, this would aid to easily control and eliminate these
bacteria, and to lower farmer's material losses as well the risk of its impact on
humans.
