Master of Biotechnology
scholar.ppu.edu/handle/123456789/42
2024-03-29T05:45:36ZIn vitro propagation of Royal Irises (section = Oncocyclus) in Palestine
scholar.ppu.edu/handle/123456789/8814
In vitro propagation of Royal Irises (section = Oncocyclus) in Palestine
Tamimi, Lana
Palestine is a region rich in plant biodiversity. Despite that, several
species are threatened due to various factors including overgrazing,
human activities and intensive agriculture. As a part of the species,
Iris species from section Oncocyclus are considered threatened
species according to the IUCN red list. As a tool to protect and
conserve these species, a protocol of in vitro propagation of
Palestinian Royal Irises is developed. This study contains four phases:
first, selection of suitable explants and establishment of irises from
the embryos on an aseptic culture. Second, in vitro shoot proliferation
and regeneration, followed by in vitro shoot multiplication. Third, in
vitro rooting. Finally, hardening of plantlets in the greenhouse
gradually. The highest embryo germination percentage was 100% on
a half-strength MS medium without PGRs. The best shoot
regeneration was obtained on half-strength MS medium supplemented
with 0.5 mg/L or 1.0 mg/L of BA while the best rooting results were
on half-strength MS medium supplemented with 0.5 mg/L IBA.
Plantlets were acclimatized in peatmoss:sand (2:1) substrate with a
survival rate of 75% - 100% after 4 weeks from transfer to pots. This
method was efficient in overcoming seed dormancy in irises.
CD, no of pages 76, biotechnology 4/2022
2022-01-01T00:00:00ZIn vitro shoot regeneration and genetic differentiation of Baladi cauliflower (Brassica oleracea var. botrytis) in Hebron
scholar.ppu.edu/handle/123456789/8727
In vitro shoot regeneration and genetic differentiation of Baladi cauliflower (Brassica oleracea var. botrytis) in Hebron
Jubeh, Haniya
Cauliflower exhibits a transition from vegetative to flowering growth triggered by
low temperature (vernalization) which controls late or early flowering. FLC gene
homologs in Brassica species controls flowering response. In this study, integrated
molecular and tissue culture approaches were used to explore genetic variation in
the flowering genes and to improved cauliflower crop. In the molecular approach,
part of the BoFLC.HP gene was sequenced in hybrid and local "Baladi" cauliflower
using the F7R7 primer to characterize early or late FLC gene in cauliflower.
Sequenced BoFLC.HP region showed a point mutation at position 91 and an indel at
position 212 to 219 in intron number 2 between Baladi and F1- hybrid cauliflower
which controls flowering time. In the tissue culture approach, hypocotyl and
cotyledon explants from four-day-old seedlings were cultured on Murashige and
Skoog (MS) salts supplemented with BA, KIN and TDZ alone or in combination with
IBA or NAA. The highest shoot regeneration response (80%) was observed in
hypocotyl explants on full strength MS media supplemented 0.5 mg/L BA and 0.5
mg/L IBA. Cotyledon explants, showed 26% shoot regeneration on full strength MS
media supplemented 2.0 mg/L BA and 0.1 mg/L NAA. One of the most important
tools in crop improvement is the process of genetic transformation with the
Agrobacterium, which requires an efficient in vitro shoot regeneration protocol from
explants. These two aspects will be a prerequisite in cauliflower crop development
in the future.
Keywords: Brassica oleracea var. botrytis, flowering time, vernalization, BoFLC.HP
gene, in vitro shoot regeneration, Hypocotyl, Cotyledon.
CD , no of pages 52, 31152, biotechnology 4/2021
2021-09-01T00:00:00ZAllele frequencies of CYP2C9, CYP2C19, and CYP2D6 in the palesinian population
scholar.ppu.edu/handle/123456789/8726
Allele frequencies of CYP2C9, CYP2C19, and CYP2D6 in the palesinian population
Wazwaz, Lamees
The polymorphism of CYP2C9, CYP2C19 and CYP2D6 differs among different individuals and
shows ethnic variability. This will affect the metabolism of several drugs and may cause
differences in the clinical response. In this study, we analyzed the genotype profile of a random
sample of the Palestinian population to compare the allele frequencies of CYP2C9, CYP2C19
and CYP2D6. Data have been taken from 67 unrelated Palestinian volunteers (35 males and 32
females, aged from 16 to 60 years). Next generation sequencing (NGS) was conducted to
sequence the most frequent variants of the CYP2C9, CYP2C19, and CYP2D6. To compare the
frequency of different variants with the global data, we used two libraries of python, i.e.
Matplotlib, and Pandas. The frequency of CYP2C9 and CYP2D6 between the Palestinian
population and the global populations shows a high correlation (0.95, and 0.96 respectively).
However, we found a low correlation for CYP2C19 (0.34). Only rs9332239 and rs28371685 in
CYP2C9 variants’ frequencies showed a significant difference between the Palestinian
population and the global population, (p-value=0.00311776) for the reference variant and (pvalue=0.003118) for the alternative variant, respectively. For CYP2C19 variants: rs28399504
and rs41291556 showed a significant difference between the Palestinian population and the
global population (p-value=0.014664745) for the reference variant and (p-value=0.014664745)
for the alternative variant. There were no statistically significant differences for all the other
variants examined for CYP2C9 (P-value = 0.847232), CYP2C19 (P-value= 0.479754), and
CYP2D6 (P-value= 0.923857). Up to our knowledge this is the first study done to compare the
Palestinian pharmacogenetics profile in those specific genes. Further studies on a larger
population sample are necessary to confirm our findings
Cd , no of pages 107, 31150 , biotechnology 2/2022
2022-05-01T00:00:00ZIdentification and Phylogenetic Analysis of Newcastle Disease Virus in the West Bank, Palestine
scholar.ppu.edu/handle/123456789/8725
Identification and Phylogenetic Analysis of Newcastle Disease Virus in the West Bank, Palestine
Seder, Rana
no of pages 65, 31151, biotechnology 3/2022
2022-04-01T00:00:00Z