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Efficient infection of cells in culture by type O foot-and-mouth disease virus requires binding to cell surface heparan sulfate

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dc.contributor.author Jackson, Terry
dc.contributor.author Ellard, Fiona
dc.contributor.author Abu Ghazaleh, Robin
dc.contributor.author Brookes, Sharon
dc.contributor.author Blakemore, Wendy
dc.contributor.author Corteyn, Amanda
dc.contributor.author Stuart, David
dc.contributor.author Newman, JW
dc.contributor.author King, AM
dc.date.accessioned 2020-11-23T07:37:07Z
dc.date.available 2020-11-23T07:37:07Z
dc.date.issued 1996-08-01
dc.identifier.citation Jackson T, Ellard FM, Ghazaleh RA, Brookes SM, Blakemore WE, Corteyn AH, Stuart DI, Newman JW, King AM. Efficient infection of cells in culture by type O foot-and-mouth disease virus requires binding to cell surface heparan sulfate. J Virol. 1996 Aug;70(8):5282-7. doi: 10.1128/JVI.70.8.5282-5287.1996. PMID: 8764038; PMCID: PMC190485. en_US
dc.identifier.uri http://scholar.ppu.edu/handle/123456789/2009
dc.description.abstract Foot-and-mouth disease virus (FMDV) enters cells by attaching to cellular receptor molecules of the integrin family, one of which has been identified as the RGD-binding integrin alpha(v)beta3. Here we report that, in addition to an integrin binding site, type O strains of FMDV share with natural ligands of alpha(v)beta3 (i.e., vitronectin and fibronectin) a specific affinity for heparin and that binding to the cellular form of this sulfated glycan, heparan sulfate, is required for efficient infection of cells in culture. Binding of the virus to paraformaldehyde-fixed cells was powerfully inhibited by agents such as heparin, that compete with heparan sulfate or by agents that compete for heparan sulfate (platelet factor 4) or that inactivate it (heparinase). Neither chondroitin sulfate, a structurally related component of the extracellular matrix, nor dextran sulfate appreciably inhibited binding. The functional importance of heparan sulfate binding was demonstrated by the facts that (i) infection of live cells by FMDV could also be blocked specifically by heparin, albeit at a much higher concentration of inhibitor; (ii) pretreatment of cells with heparinase reduced the number of plaques formed compared with that for untreated cells; and (iii) mutant cell lines deficient in heparan sulfate expression were unable to support plaque formation by FMDV, even though they remained equally susceptible to another picornavirus, bovine enterovirus. The results show that entry of type O FMDV into cells is a complex process and suggest that the initial contact with the cell surface is made through heparan sulfate. en_US
dc.language.iso en en_US
dc.publisher American Society for Microbiology en_US
dc.title Efficient infection of cells in culture by type O foot-and-mouth disease virus requires binding to cell surface heparan sulfate en_US
dc.type Article en_US


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