Abstract:
Rhinoviruses (RVs) are classified in the Enteroviruses genus of Picornaviridae family. RVs are a major
cause of the common cold. Several studies have shown that the RV is associated with other respiratory
diseases such as asthma and sinusitis in young children. RVs are highly communicable viruses,
particularly during the spring and fall months. RVs are divided into three genetic diversity groups (A, B,
and C) with more than 160 serotypes. In Palestine and other parts of the Middle East, the epidemiology
of RVs is not well defined.
Objective
This study examines the molecular epidemiology of RV in patients admitted to Caritas Baby Hospital
(CBH) during 2013 and evaluates the co-infection rates between RVs and other common respiratory
viruses.
Materials and Methods
Nasopharyngeal aspirates (N=1,745) collected from patients admitted to CBH in 2013 with upper
respiratory tract infection were tested by Direct Fluorescent Antibody (DFA) staining for eight common
human respiratory viruses (influenza viruses A and B, respiratory syncytial virus (RSV), human
metapneumovirus (hMPV), parainfluenza 1, 2, and 3, and adenovirus). Nasopharyngeal aspirates
collected from patients less than 14 years of age between January and December 2013 where included in
the study. Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) was used to
evaluate the presence of RVs in the DFA tested samples. In addition, RV subgrouping (A, B, or C) was
performed on 184 (26.3%) RV positive patients samples by RT-PCR. Finally, sequence analysis of
partially amplified RV genome sequences were performed on different RV subgroups on positive
patient's samples and used to determine the RV genotypes.
Description:
Cd , No of pages 65,Biotechnology 1/2018 , 31011 , master
